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Product Description

Catalogue Number: B688500
Chemical Name: Bromotrimethylammoniumbimane Bromide
Synonyms: 5-(Bromomethyl)-N,N,N,2,6-pentamethyl-1,7-dioxo-1H,7H-pyrazolo[1,2-a]pyrazole-3-methanaminium Bromide; Bromobimane q; Thiolyte MQ;
CAS Number: 71418-45-6
Molecular Formula: C₁₃H₁₉Br₂N₃O₂
Appearance: Pale Yellow to Yellow Solid
Melting Point: >170°C (dec.)
Molecular Weight: 409.12
Storage: Amber Vial, -20°C Freezer, Under inert atmosphere
Solubility: Methanol (Slightly), Water (Slightly)
Stability: Moisture, Light Sensitive - Desiccate and protect from light
Category: Standards; Fluorescent Reagents; Sulfhydryl Active Reagents;
Applications: A charged fluoresent sulfhydryl active reagent. Bromotrimethylammoniumbimane is a derivative of monobromobimane containing a positive charge which permits separation of its conjugates by electrophoresis or cation-exchange chromatography. The membrane permeability of this charged bimane as well as its ability to access sites within a protein may differ from that of the uncharged bimane probes. These reagents are also useful for detecting the distribution of protein thiols in cells before and after chemical reduction with disulfides. Fluorescence: max. Abs. l = 398nm, max. Em. l = 475-485 (when reacted with thiol); e x 10-3: 5.0
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