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Product Description

Catalogue Number:A634000
Chemical Name:Ammonium 7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate
Synonyms:Ammonium 7-Fluorobenz-2-oxa-1,3-diazole-4-sulfonate; 7-Fluorobenzofurazan-4-sulfonic Acid Ammonium Salt; SBD-F
CAS Number:84806-27-9
Alternate CAS #:Free acid 91575-54-1
Molecular Formula:C₆H₆FN₃O₄S
Appearance:Light Yellow to Orange Solid
Melting Point:>260°C dec.
Molecular Weight:235.2
Storage:Amber Vial, -20°C Freezer, Under Inert Atmosphere
Solubility:Ethanol (Slightly), Methanol (Slightly), Water (Slightly)
Stability:Light Sensitive, Hygroscopic
Category: Research Tools; Molecular Biology; Fluorescent Reagents; Sulfhydryl Active Reagents;
Applications:A water soluble bifunctional fluorogenic reagent for the sensitive and specific detection of thiols in biological specimens or proteins. SBD-F has high reactivity to thiol compounds to attain low detection limits; no flourescence in itself; biological buffer solublility; stability at RT in water for >1 week. The optimal condition for reaction was 60oC and pH 9.5 for 1 h. Please refer to the following references for more specific information regarding the application of SBD-F as a fluorogenic reagent for detection of cysteine containing peptides. Fluorescence: max Abs. l = 380 nm, max Em. l = 515 nm; Molar Abs. > 4x103 (ca. 317nm)