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50μg
$ 170.00 0.0
100μg
$ 245.00 0.0
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Product Description

Catalogue Number: AN00008
Product Name: anti- 6*His, His-Tag antibody
Form: liquid
Host: Mouse
Clonity: monoclonal
Isotope: IgG1
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.
Synonyms: 6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, HIS, His Tag, HISTIDINE TAGGED
Specificity: Recombinant protein
Tested Application: ELISA, WB, IP, IF
Recommended Dilution: WB : 1:1000-1:10000;IP : 1:1000-1:10000;IF : 1:20-1:100
WB Dilution: Western blot of 6*His-tagged fusion protein with anti-6*HIS tag at dilution of 1:5000.