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|Product Name:||anti- 4EBP1 antibody|
|Storage:||PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)|
|Background:||Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.|
|Immunogen:||eukaryotic translation initiation factor 4E binding protein 1|
|Synonyms:||4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I|
|Tested Application:||ELISA, WB, IHC|
|Recommended Dilution:||WB : 1:1000-1:10000;IHC : 1:20-1:200;IF : 1:20-1:200|
|IHC Dilution:||Immunohistochemistry of paraffin-embedded human pancreas slide using 4EBP1 Antibody at dilution of 1:50|
|WB Dilution:||K-562 cells were subjected to SDS PAGE followed by western blot with 4EBP1 antibody at dilution of 1:2000|