Rapid screening of Alternaria mycotoxins using MALDI-TOF mass spectrometry.
Sivagnanam K, Komatsu E, Rampitsch C, Perreault H, Grafenhan T
Members of the Alternaria genus produce various toxins and their occurrence in agricultural commodities is a major concern for humans and the environment. The present study developed a simple and efficient MALDI-TOF MS method for the rapid detection of Alternaria toxins. A new method for the detection of alternariol (AOH), alternariol monomethyl ether (AME), and tentoxin (TEN) was developed by matrix assisted laser desorption ionization - time of flight (MALDI-TOF) mass spectrometry (MS). Different solid phase extraction (SPE) clean-up method were tried to optimize the purification of wheat matrix and an optimum extraction method was designed to recover the three Alternaria toxins. In addition, various MALDI matrices were examined and ?-cyano-4-hydroxycinnamic acid (CHCA) matrix gave a good repeatability for all three Alternaria toxins. This is the first study to report the detection of three important Alternaria toxins concurrently using MALDI-TOF MS and this study opens up the possibility of rapid screening of Alternaria toxins in several other cereals and food products.
Alternaria; MALDI-TOF; cereal grains; mass spectrometry; mycotoxins