Ethidium Bromide

Catalogue number: E890700
Chemical name: Ethidium Bromide
Synonyms: 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide; 2,7-Diamino-10-ethyl-9- phenylphenanthridinium Bromide; 2,7-Diamino-9-phenyl-10-ethylphenanthridinium Bromide; 2,7-Diamino-9-phenylphenanthridine Ethobromide; Dromilac; Homidium Bromide;
CAS Number: 1239-45-8
Possible CAS #: NA
Molecular form.: C₂₁H₂₀BrN₃
Appearance: Very Dark Red to Dark Purple Solid
Melting Point: 242-244°C
Mol. Weight: 394.31
Storage: Hygroscopic, Refrigerator, under inert atmosphere
Solubility: DMSO (Slightly), Methanol (Slightly), Water (Slightly, Sonicated)
Stability: No Data Available
Category: Amines, Aromatics, Pharmaceuticals, Intermediates & Fine Chemicals
Boiling Point: No Data Available
Applications: Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
Dangerous Goods Info: NA
References: Waring, M.J., et al.: J. Mol. Biol., 13, 269 (1965), Probst, G.S., et al.: Environ. Mutagen., 3, 11 (1981),
Peer Review Product Citations:

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